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Issue 3, 2013
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1H NMR-based metabolomics exploring biomarkers in rat cerebrospinal fluid after cerebral ischemia/reperfusion

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Abstract

In our study, metabolomics was used to investigate biochemical changes in the early stages of rats focal cerebral ischemia/reperfusion (I/R) injury. Cerebrospinal fluid (CSF) samples at 0, 0.5, 1, 3, and 6 h of reperfusion (n = 10), based on 1H NMR spectroscopy and multivariate data analyses, were tested to analyze the changing of metabolites during the early disease process. Partial least squares-discriminant analysis scores plots of the 1H NMR data revealed clear differences among the experiment groups. Combining the results of the loading plot and t-test, we found that twenty-seven metabolites were changed significantly (p < 0.05) in the CSF samples among the different groups. Among that, the potential biomarkers in CSF of ischemic rats were: acetic acid, 3-hydroxyisovaleric acid, 3-hydroxybutyric acid, choline, L-alanine, creatine, creatinine, glycine, pyruvic acid, glycerol, glutamic acid, D-fructose, L-lactic acid and acetone. These findings help us understand the biochemical metabolite changes in CSF of I/R rats in early stages. What's more, metabolomics may, therefore, have the potential to be developed into a clinically useful diagnostic tool of ischemic brain injury.

Graphical abstract: 1H NMR-based metabolomics exploring biomarkers in rat cerebrospinal fluid after cerebral ischemia/reperfusion

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Supplementary files

Article information


Submitted
07 Jun 2012
Accepted
07 Dec 2012
First published
14 Dec 2012

Mol. BioSyst., 2013,9, 431-439
Article type
Paper

1 H NMR-based metabolomics exploring biomarkers in rat cerebrospinal fluid after cerebral ischemia/reperfusion

Y. Wang, Y. Wang, M. Li, P. Xu, T. Gu, T. Ma and S. Gu, Mol. BioSyst., 2013, 9, 431
DOI: 10.1039/C2MB25224D

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