Issue 2, 2013
From the journal:

Green Chemistry

More efficient redox biocatalysis by utilising 1,4-butanediol as a ‘smart cosubstrate’†

Selin Kara,a   Dominik Spickermann,b   Joerg H. Schrittwieser,a   Christian Leggewie,b   Willem J. H. van Berkel,c   Isabel W. C. E. Arendsa  and  Frank Hollmann*a  

* Corresponding authors

a Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628BL Delft, The Netherlands
E-mail: f.hollmann@tudelft.nl
Fax: (+31) 152 781415
Tel: (+31) 152 781957

b evocatal GmbH, Merowingerplatz 1A, 40225 Düsseldorf, Germany

c Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands

Abstract

1,4-Butanediol is shown to be an efficient cosubstrate to promote NAD(P)H-dependent redox biocatalysis. The thermodynamically and kinetically inert lactone coproduct makes the regeneration reaction irreversible. Thereby not only the molar surplus of cosubstrate is dramatically reduced but also faster reaction rates are obtained.

Graphical abstract: More efficient redox biocatalysis by utilising 1,4-butanediol as a ‘smart cosubstrate’

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Article information

DOI
https://doi.org/10.1039/C2GC36797A
Article type
Communication
Submitted
12 Nov 2012
Accepted
19 Dec 2012
First published
20 Dec 2012
This article is Open Access
Creative Commons BY license

Green Chem., 2013,15, 330-335

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More efficient redox biocatalysis by utilising 1,4-butanediol as a ‘smart cosubstrate’

S. Kara, D. Spickermann, J. H. Schrittwieser, C. Leggewie, W. J. H. van Berkel, I. W. C. E. Arends and F. Hollmann, Green Chem., 2013, 15, 330 DOI: 10.1039/C2GC36797A

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