Jump to main content
Jump to site search

Issue 50, 2013
Previous Article Next Article

Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

Author affiliations

Abstract

We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.

Graphical abstract: Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Feb 2013, accepted on 26 Mar 2013 and first published on 26 Mar 2013


Article type: Communication
DOI: 10.1039/C3CC41129J
Citation: Chem. Commun., 2013,49, 5630-5632

  •   Request permissions

    Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe

    K. Leung, H. He, V. P. Ma, H. Zhong, D. S. Chan, J. Zhou, J. Mergny, C. Leung and D. Ma, Chem. Commun., 2013, 49, 5630
    DOI: 10.1039/C3CC41129J

Search articles by author

Spotlight

Advertisements