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Issue 41, 2013
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Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling†

Petra Ménová,a   Hana Cahová,a   Medard Plucnara,b   Luděk Havran,b   Miroslav Fojta*bc  and  Michal Hocek*ad  
Author affiliations

* Corresponding authors

a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead & IOCB Research Center, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic
E-mail: hocek@uochb.cas.cz

b Institute of Biophysics, v.v.i. Academy of Sciences of the Czech Republic, Kralovopolska 135, 61265 Brno, Czech Republic
E-mail: fojta@ibp.cz

c Central European Institute of Technology, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech Republic

d Department of Organic and Nuclear Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic

Abstract

Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.

Graphical abstract: Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

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Publication details

The article was received on 25 Feb 2013, accepted on 02 Apr 2013 and first published on 03 Apr 2013


Article type: Communication
DOI: 10.1039/C3CC41438H
Chem. Commun., 2013,49, 4652-4654
  • Open access: Creative Commons BY-NC license
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    Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

    P. Ménová, H. Cahová, M. Plucnara, L. Havran, M. Fojta and M. Hocek, Chem. Commun., 2013, 49, 4652
    DOI: 10.1039/C3CC41438H

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