Jump to main content
Jump to site search

Issue 9, 2013
Previous Article Next Article

Determination of triazolepesticides in rat blood by the combination of ultrasound-enhanced temperature-controlled ionic liquid dispersive liquid–liquid microextraction coupled to high-performance liquid chromatography

Author affiliations

Abstract

This paper presents a study of the performance of ultrasound-enhanced temperature-controlled (UETC) ionic liquid dispersive liquid–liquid microextraction (IL-DLLME). The use of ultrasonication and heating can enhance the ability of the ionic liquid to extract the analytes. Various parameters that would affect the extraction efficiency, such as the type and volume of the extraction and dispersing solvents, salt concentration, pH value, centrifugation time, effect of temperature on UETC-IL-DLLME, were investigated and optimized. Under the optimal extraction conditions, good enrichment factors (178–197) and recoveries (88.9–98.5%) were obtained. Good linearity was obtained in the range of 4–500 μg L−1 for myclobutanil and in the range of 6–500 μg L−1 for uniconazole, penconazole, tebuconazole and hexaconazole. Based on the optimized conditions, the UETC-IL-DLLME method was applied and combined with high-performance liquid chromatography with diode array detection (HPLC-DAD) to determine the presence of five triazole fungicides. The results showed that the method we proposed could effectively determine the target fungicides in rat blood samples.

Graphical abstract: Determination of triazole pesticides in rat blood by the combination of ultrasound-enhanced temperature-controlled ionic liquid dispersive liquid–liquid microextraction coupled to high-performance liquid chromatography

Back to tab navigation

Article information


Submitted
18 Jun 2012
Accepted
10 Feb 2013
First published
11 Feb 2013

Anal. Methods, 2013,5, 2241-2248
Article type
Paper

Determination of triazole pesticides in rat blood by the combination of ultrasound-enhanced temperature-controlled ionic liquid dispersive liquid–liquid microextraction coupled to high-performance liquid chromatography

Y. Li, J. Zhang, B. Peng, S. Li, H. Gao and W. Zhou, Anal. Methods, 2013, 5, 2241
DOI: 10.1039/C3AY25626J

Social activity

Search articles by author

Spotlight

Advertisements