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Issue 3, 2012
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Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

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Abstract

We developed a promising candidate for a hypersensitive magnetic resonance (MR) reporter protein. The reporter protein is comprised of mouse lactate dehydrogenase-X (mLDH-X) and a hyperpolarized pyruvic acid derivative, substrate Y that gives no false-positive signals by endogenous human lactate dehydrogenases (hLDHs). The X–Y pair shows high MR sensitivity and sufficient reaction kinetics, thus enabling direct detection of reporter protein X in crude biological mixtures by monitoring the 13C NMR signal of hyperpolarized product Yr.

Graphical abstract: Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

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Supplementary files

Article information


Submitted
14 Nov 2011
Accepted
15 Nov 2011
First published
01 Dec 2011

Chem. Sci., 2012,3, 800-806
Article type
Edge Article

Mouse lactate dehydrogenase X: A promising magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative Y

T. Nishihara, H. Nonaka, T. Naganuma, K. Ichikawa and S. Sando, Chem. Sci., 2012, 3, 800
DOI: 10.1039/C1SC00919B

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