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Issue 18, 2011
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LC-MS based quantification of 2′-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

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Abstract

RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2′-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi.

Graphical abstract: LC-MS based quantification of 2′-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

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Supplementary files

Article information


Submitted
21 Feb 2011
Accepted
13 Mar 2011
First published
29 Mar 2011

Chem. Commun., 2011,47, 5196-5198
Article type
Communication

LC-MS based quantification of 2′-ribosylated nucleosides Ar(p) and Gr(p) in tRNA

D. Pearson, A. Hienzsch, M. Wagner, D. Globisch, V. Reiter, D. Özden and T. Carell, Chem. Commun., 2011, 47, 5196
DOI: 10.1039/C1CC11011J

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