Engineering of a redox protein for DNA-directed assembly

Géza R. Szilvay; Shayna Brocato; Dmitri Ivnitski; Carol Li; Pablo De La Iglesia; Carolin Lau; Eva Chi; Margaret Werner-Washburne; Scott Banta; Plamen Atanassov

doi:10.1039/C1CC11951F

Engineering of a redox protein for DNA-directed assembly†

Géza R. Szilvay,‡^a Shayna Brocato,^b Dmitri Ivnitski,^b Carol Li,^a Pablo De La Iglesia,^b Carolin Lau,^b Eva Chi,^b Margaret Werner-Washburne,^c Scott Banta^a and Plamen Atanassov*^b

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^a Department of Chemical Engineering, Columbia University, 500 W 120th Street, New York, USA
E-mail: sb2373@columbia.edu, geza.szilvay@vtt.fi
Fax: +1 212-854-3054
Tel: +1 2128547531

^b Department of Chemical and Nuclear Engineering, University of New Mexico, 1 University of New Mexico, Albuquerque, USA
E-mail: plamen@unm.edu
Fax: +1 505-277-5433
Tel: +1 505-277-2640

^c Department of Biology, University of New Mexico, 1 University of New Mexico, Albuquerque, USA
E-mail: maggieww@unm.edu
Tel: +1 505-277-9338

Abstract

Engineered enzyme conjugate of the small laccase enzyme from Streptomyces coelicolor and zinc finger DNA binding domain from Zif268 is demonstrated to bind double stranded DNA in a site specific manner while retaining enzymatic activity.

Chemical Communications

Engineering of a redox protein for DNA-directed assembly†

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Engineering of a redox protein for DNA-directed assembly

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