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Issue 7, 2010
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Analytical strategies for detecting nanoparticleprotein interactions

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A significant increase in biomedical applications of nanomaterials and their potential toxicity demands versatile analytical techniques to determine proteinnanoparticle (NP) interactions. These diverse analytical techniques are reviewed. Spectroscopic methods play a significant role in studying binding affinity, binding ratio, and binding mechanisms. To elucidate NP–proteome interactions, chromatography and electrophoresis techniques are applied to separate NP-bound proteins and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to identify these proteins. Since NP–protein binding is a dynamic event, surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) are methods of choice to study the kinetics of NP–protein binding.

Graphical abstract: Analytical strategies for detecting nanoparticle–protein interactions

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Publication details

The article was received on 08 Feb 2010, accepted on 25 Mar 2010 and first published on 26 May 2010

Article type: Critical Review
DOI: 10.1039/C0AN00075B
Citation: Analyst, 2010,135, 1519-1530
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    Analytical strategies for detecting nanoparticleprotein interactions

    L. Li, Q. Mu, B. Zhang and B. Yan, Analyst, 2010, 135, 1519
    DOI: 10.1039/C0AN00075B

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