Dual colour, microarray-based, analysis of 10 000 protease substrates

Juan J. Díaz-Mochón; Laurent Bialy; Mark Bradley

doi:10.1039/B609029J

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Dual colour, microarray-based, analysis of 10 000 protease substrates†

Juan J. Díaz-Mochón,^a Laurent Bialy^a and Mark Bradley*^a

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* Corresponding authors

^a University of Edinburgh, School of Chemistry, Joseph Black Buildings, Midlothian, UK
E-mail: mark.bradley@ed.ac.uk
Fax: +44(0)1316506453

Abstract

A 10 000 member PNA-encoded library of FRET based peptides was synthesised for global analysis of protease cleavage specificity; analysis was achieved using a DNA microarray and consumed minimal quantities of enzyme (60 pmole) and library (3.5 nmole).

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Supplementary files

Experimental details; PNA encoding triplets with their corresponding amino acids; DNA oligomers used on OGT arrays; parameters used for data analysis and full data PDF (163K)

Article information

DOI: https://doi.org/10.1039/B609029J
Article type: Communication
Submitted: 26 Jun 2006
Accepted: 22 Aug 2006
First published: 08 Sep 2006

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Chem. Commun., 2006, 3984-3986

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Dual colour, microarray-based, analysis of 10 000 protease substrates

J. J. Díaz-Mochón, L. Bialy and M. Bradley, Chem. Commun., 2006, 3984 DOI: 10.1039/B609029J

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