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Issue 2, 2006
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Nanometre-sized molecular oxygen sensors prepared from polymer stabilized phospholipid vesicles

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Abstract

Nanometre-sized, chemically-stabilized phospholipid vesicle sensors have been developed for detection of dissolved molecular oxygen. Sensors were prepared by forming 150 nm phospholipid vesicles from 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or DOPC doped with small (<1%) mole percentages of 1,2-dioleoyl-sn-glycero-3-phosphoethanol amine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE). Sensors were stabilized via cross-linking polymerization of hydrophobic methacrylate monomers partitioned into the hydrophobic interior of the DOPC bilayer. The resultant unilamellar, nanometre-sized, polymerlipid vesicles are spherical, biocompatible and protect sensing components that are loaded into the aqueous interior of the vesicle from interfering species in the exterior environment. For O2 detection, the oxygen-sensitive fluorescent dye, tris(1,10-phenanthroline)ruthenium(II) chloride (Ru(phen)3) was encapsulated into the aqueous interior of the polymerized phospholipid vesicle. NBD-PE was introduced into the phospholipid bilayer of the sensor as a reference dye, allowing ratiometric sensors to be constructed. The resultant sensors show high sensitivity, excellent reversibility and excellent linearity over a physiological range of dissolved oxygen concentrations. These results suggest that polymerized phospholipid vesicle sensors can be used for monitoring intracellular O2 dynamics.

Graphical abstract: Nanometre-sized molecular oxygen sensors prepared from polymer stabilized phospholipid vesicles

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Supplementary files

Article information


Submitted
04 Aug 2005
Accepted
28 Nov 2005
First published
14 Dec 2005

Analyst, 2006,131, 236-243
Article type
Paper

Nanometre-sized molecular oxygen sensors prepared from polymer stabilized phospholipid vesicles

Z. Cheng and C. A. Aspinwall, Analyst, 2006, 131, 236
DOI: 10.1039/B511083A

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