Issue 6, 2003

Acoustic wave detection of chemical species electrokinetically transported within a capillary tube

Abstract

For the first time, we report the acoustic wave detection of chemical species being transported in a capillary tube to a region where acoustic coupling occurs. The measured parameter was a change in phase, which was originally only attributed to a change in solution density as the analyte passed by the detection region. Accordingly, we report the detection of change in phase as various chemical species (e.g. Cy5 dye, Cy5-derivatized glycine and underivatized glycine) were introduced into and migrated along a capillary tube through electrokinetic processes. To improve detection sensitivity, we modified various experimental parameters, such as run buffer concentration, capillary wall thickness and transducer frequency. Although acoustic wave detection was feasible, the peak width and detection limit were inadequate as compared to conventional detection methods for HPLC or CE. Nevertheless, the effects of various physical and chemical relaxation processes on acoustic wave absorption were discussed, and this has shed some light on explaining some observations, which cannot be explained by density differences alone. Accordingly, the acoustic wave method is suggested to investigate these processes, as studied in ultrasonic relaxation spectroscopy, in a flow system.

Article information

Article type
Paper
Submitted
19 Dec 2002
Accepted
16 Apr 2003
First published
06 May 2003

Analyst, 2003,128, 706-711

Acoustic wave detection of chemical species electrokinetically transported within a capillary tube

P. C. H. Li and R. Prasad, Analyst, 2003, 128, 706 DOI: 10.1039/B212492K

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