Issue 5, 2000

Potentiality of proton nuclear magnetic resonance and multivariate calibration methods for the determination of dermatan sulfate contamination in heparin samples

Abstract

A 1H NMR method for the quantification of dermatan sulfate impurities in heparin industrial samples is proposed. The method is based on the analysis of 1H NMR spectral data by multivariate calibration. The 1H NMR spectra of heparin and dermatan sulfate standards showed characteristic profiles. Thus, differences in the methyl peaks of acetamido groups of heparin and dermatan sulfate were greatly advantageous for the analysis. Other hydrogens of the sugar ring were also relevant in this study. Thus, the determination of dermatan sulfate by multivariate calibration depended on all these differences. Partial least squares regression (PLS) was chosen as the calibration method. In addition, a data standardization procedure was developed in order that 1H NMR spectra registered with different instruments operating under different measurement conditions were comparable. The quantification of dermatan sulfate in the samples was satisfactory, with an overall prediction error of 6%.

Article information

Article type
Paper
Submitted
27 Jan 2000
Accepted
21 Mar 2000
First published
18 Apr 2000

Analyst, 2000,125, 933-938

Potentiality of proton nuclear magnetic resonance and multivariate calibration methods for the determination of dermatan sulfate contamination in heparin samples

V. Ruiz-Calero, J. Saurina, M. T. Galceran, S. Hernández-Cassou and L. Puignou, Analyst, 2000, 125, 933 DOI: 10.1039/B000768O

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements