A novel screening method for combinatorial chemistry was developed by utilizing the high binding affinity of the avidin–biotin complex (Ka = 1015 M–1), making possible the efficient detection of low affinity interactions between tryptophan and pentapeptides.
N. Sugimoto, H. Kazuta, J. Zou and D. Miyoshi, Chem. Commun., 1999, 677 DOI: 10.1039/A809090D
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