Study of protolytic, hydrolytic and solubility equilibria of midazolam

Abstract

Protolytic equilibria of midazolam in the pH range 0–12 were investigated. Acidity, hydrolysis, equilibrium constants in the heterogeneous system and the solubility of midazolam were determined [I= 0.1 mol l^–1(NaCl), 25 °C]. A spectrophotometric method was developed for the investigation of the mechanism of hydrolysis and the determination of the corresponding equilibrium constants. In addition, buffer characteristics of the two-phase and single-phase midazolam buffers were investigated.