The binding of Hoechst 33258 to the minor groove of the duplex formed by the self-complementary oligonucleotide d(CGCGAATTCGCG) has been shown by means of high-field 1H nuclear magnetic resonance spectroscopy to be across the central AATT region of the duplex.
J. A. Parkinson, J. Barber, K. T. Douglas, J. Rosamund and D. Sharples, J. Chem. Soc., Chem. Commun., 1989, 1023 DOI: 10.1039/C39890001023
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