Horseradish peroxidase assay: optimisation of carbon–fluoride bond breakage and characterisation of its kinetics using the fluoride ion-selective electrode

Abstract

The horseradish peroxidase catalysed breakage of the carbon–fluoride bond in the two organofluorides, 4-fluorophenol and 2,3,4,5,6-pentafluorophenol, have been compared and optimised using the fluoride ion-selective electrode. 4-Fluorophenol was found to have a higher rate of fluoride ion release than 2,3,4,5,6-pentafluorophenol. The reaction of 4-fluorophenol was optimised further for pH and temperature. By measuring the rate of fluoride ion release for a nine by eleven matrix (corresponding to concentrations of 4-fluorophenol and hydrogen peroxide, respectively) a three-dimensional model has been developed.