Issue 11, 1989

Analysis of basic drugs by high-performance liquid chromatography using on-line solid-phase extraction and an unmodified silica column

Abstract

A method is described for the high-performance liquid chromatographic analysis of a number of basic drugs on unmodified silica gel, which employs an aqueous methanol-rich eluent of pH 8. The analytical system was coupled to a column-switching assembly in order to facilitate on-line solid-phase extraction of the drugs from plasma. The method is reproducible, with a coefficient of variation of 3% or less for most compounds, even without internal standardisation. Drug recovery from plasma is efficient, being in excess of 90% in most instances. The drugs were detected by UV absorption at 254 nm.

Article information

Article type
Paper

Analyst, 1989,114, 1377-1380

Analysis of basic drugs by high-performance liquid chromatography using on-line solid-phase extraction and an unmodified silica column

M. T. Kelly, M. R. Smyth and D. Dadgar, Analyst, 1989, 114, 1377 DOI: 10.1039/AN9891401377

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