Potentiometric monitoring of proteins. Part IV. Application to electrophoresis gels

Abstract

A simple method for the determination of proteins separated by gel electrophoresis is described based on direct potentiometry with copper electrodes. Results are presented for a number of proteins in agarose gels. Several different electrode designs are considered and the effects of various buffer systems are investigated. It is shown that cathodic pre-treatment of the monitoring electrodes gives a fast and reproducible response which is sufficient to form the basis of a simple, reliable and inexpensive method for the quantitative monitoring of proteins in electrophoresis gels.