A rapid, highly sensitive and specific method for the determination of ergosterol in food grains contaminated with fungi is described. The method involves simple iodination of ergosterol resulting in the formation of a highly fluorescent product which is determined fluorodensitometrically. The method may find wide application for the screening of food grains contaminated with fungi.
R. B. Sashidhar, R. V. Sudershan, Y. Ramakrishna, S. Nahdi and R. V. Bhat, Analyst, 1988, 113, 809 DOI: 10.1039/AN9881300809
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