Two-dimensional heteronuclear Overhauser effect n.m.r. spectroscopy is shown to be practical with a fluorine-labelled protein; the experiment reveals through-space proton–fluorine interactions at the active site of an acylated form of α-chymotrypsin.
S. J. Hammond, J. Chem. Soc., Chem. Commun., 1984, 712 DOI: 10.1039/C39840000712
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