Improved deprotection in solid phase peptide synthesis: deprotection of Ni-formyl-tryptophan to tryptophan in low concentrations of HF in Me2S–p- thiocresol mixtures

Abstract

The Nⁱ-formy protecting group of tryptophan in solid phase peptide synthesis was found to be removed efficiently and quantitatively by a new reagent, HF–Me₂S–p-thiocresol–p-cresol (25: 65: 5: 5, v/v/v/v) without detectable side reaction.