A polarographic and ultraviolet spectral investigation of the pharmacologically active 1-benzhydryl-4-(6-methyl-2-pyridylmethyleneimino)-piperazine

Abstract

This paper presents a detailed study of the ultraviolet spectral and polarographic behaviour of a pharmacologically active benzhydrylpiperazine derivative. It explains the contribution made by various parts of the molecule to the ultraviolet spectrum and predicts the different forms of the molecule existing in aqueous media. The molecule was found to reduce in a 4e process when the nitrogen atom at position 4 became protonated and in a 2e process otherwise. This information, along with distribution studies in aqueous and biological media, made it possible to develop a polarographic method for measuring the concentrations of this molecule in plasma and urine following therapeutic administrations to test animals.