Synthesis of nucleoside di- and tri-phosphates via nucleoside 5′-phosphoric di-n-butylphosphinothioic anhydride intermediates

Abstract

Reaction of di-n-butylphosphinothioyl bromide with nucleotides afforded almost quantitatively the corresponding nucleoside phosphoric di-n-butylphosphinothioic anhydrides, which are quite stable even in the presence of water; in the presence of AgOAc they react readily with inorganic phosphate or pyrophosphate to give the corresponding nucleoside di- or tri-phosphates respectively.