Determination of riboflavine in injections containing liver extract

Abstract

Interference by the pigments of liver extract in the spectrophotometric determination of riboflavine by the method of the British Pharmacopoeia 1968 is obviated by chromatography on a column of activated acidic alumina with the mixture chloroform-ethanol (98 per cent.)-glacial acetic acid (50 + 50 + 0·3 v/v); riboflavine passes through the column while liver pigments are retained. The eluate is concentrated by evaporation, buffered at pH 4, then measured spectrophotometrically at wavelength 444 nm.