The assay of potassium 6-[L-(+)-α-phenoxypropionamido]penicillanate and 6-[D-(+)-α-phenoxypropionamido]penicillanate in phenethicillin potassium by circular dichroism spectrometry

Abstract

Circular dichroism spectra of potassium 6-[L-(+)-α-phenoxypropionamido]penicillanate and 6-[D-(+)-α-phenoxypropionamido]penicillanate have been recorded, and form the basis of an assay procedure with which to determine the proportions of these two isomers in Phenethicillin Potassium. Eight commercial samples of Phenethicillin Potassium have been assayed by this method and the results are compared with those obtained by microbiological assay