This study demonstrates the screening of a collection of twelve 19F-tagged metal-binding pharmacophores (MBPs) against the Zn(II)-dependent metalloenzyme human carbonic anhydrase II (hCAII) by 19F NMR. The isomorphous replacement of Zn(II) by Co(II) in hCAII produces enhanced sensitivity and reveals the potential of 19F NMR-based techniques for metalloenzyme ligand discovery.
K. E. Prosser, A. J. Kohlbrand, H. Seo, M. Kalaj and S. M. Cohen, Chem. Commun., 2021, 57, 4934 DOI: 10.1039/D1CC01231B
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