Issue 92, 2015
From the journal:

Chemical Communications

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(ii) drug to probe its distribution and state in vitro†

Ronald F. S. Lee,a   Stéphane Escrig,b   Marie Croisier,c   Stéphanie Clerc-Rosset,c   Graham W. Knott,c   Anders Meibom,bd   Curt A. Davey,e   Kai Johnssona  and  Paul J. Dyson*a  

* Corresponding authors

a Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland
E-mail: paul.dyson@epfl.ch
Tel: +41 21 693 98 54

b Laboratory for Biological Geochemistry, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland
E-mail: anders.meibom@epfl.ch
Tel: +41 21 693 80 15

c Interdisciplinary Centre for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland
E-mail: graham.knott@epfl.ch
Tel: +41 21 693 0962

d Center for Advanced Surface Analysis, Institute of Earth Sciences, University of Lausanne, CH-1015 Lausanne, Switzerland

e Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore

Abstract

The in vitro inter- and intra-cellular distribution of an isotopically labelled ruthenium(II)–arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of 13C, 15N, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(II) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

Graphical abstract: NanoSIMS analysis of an isotopically labelled organometallic ruthenium(ii) drug to probe its distribution and state in vitro

About
Cited by
Related
Download options Please wait...

Associated articles

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC06983A
Article type
Communication
Submitted
19 Aug 2015
Accepted
23 Sep 2015
First published
23 Sep 2015
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 16486-16489

Author version available

Download author version (PDF)

Permissions

Request permissions

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

R. F. S. Lee, S. Escrig, M. Croisier, S. Clerc-Rosset, G. W. Knott, A. Meibom, C. A. Davey, K. Johnsson and P. J. Dyson, Chem. Commun., 2015, 51, 16486 DOI: 10.1039/C5CC06983A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements