Issue 51, 2022

Hydride state accumulation in native [FeFe]-hydrogenase with the physiological reductant H2 supports its catalytic relevance

Abstract

Small molecules in solution may interfere with mechanistic investigations, as they can affect the stability of catalytic states and produce off-cycle states that can be mistaken for catalytically relevant species. Here we show that the hydride state (Hhyd), a proposed central intermediate in the catalytic cycle of [FeFe]-hydrogenase, can be formed in wild-type [FeFe]-hydrogenases treated with H2 in absence of other, non-biological, reductants. Moreover, we reveal a new state with unclear role in catalysis induced by common low pH buffers.

Graphical abstract: Hydride state accumulation in native [FeFe]-hydrogenase with the physiological reductant H2 supports its catalytic relevance

Supplementary files

Article information

Article type
Communication
Submitted
02 Feb 2022
Accepted
30 Mai 2022
First published
30 Mai 2022
This article is Open Access
Creative Commons BY license

Chem. Commun., 2022,58, 7184-7187

Hydride state accumulation in native [FeFe]-hydrogenase with the physiological reductant H2 supports its catalytic relevance

M. Senger, T. Kernmayr, M. Lorenzi, H. J. Redman and G. Berggren, Chem. Commun., 2022, 58, 7184 DOI: 10.1039/D2CC00671E

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