A bioorthogonal ‘turn-on’ fluorescent probe for tracking mitochondrial nitroxyl formation

Kyoung Sunwoo; Kondapa Naidu Bobba; Ja-Yun Lim; Taegun Park; Arup Podder; June Seok Heo; Seung Gwan Lee; Sankarprasad Bhuniya; Jong Seung Kim

doi:10.1039/C6CC09082F

A bioorthogonal ‘turn-on’ fluorescent probe for tracking mitochondrial nitroxyl formation†

Kyoung Sunwoo,‡^a Kondapa Naidu Bobba,‡^b Ja-Yun Lim,‡^c Taegun Park,

^c Arup Podder,^b June Seok Heo,^c Seung Gwan Lee,*^d Sankarprasad Bhuniya

*^be and Jong Seung Kim

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, Korea University, Seoul 136-701, Korea
E-mail: jongskim@korea.ac.kr

^b Amrita Centre for Industrial Research & Innovation, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Ettimadai, Coimbatore 641112, India
E-mail: b_sankarprasad@cb.amrita.edu

^c Department of Integrated Biomedical and Life Sciences, College of Health Science, Korea University, Seoul 136-701, Korea

^d Department of Health and Environmental Science, College of Health Science, Korea University, Seoul 136-701, Korea
E-mail: seunggwan@korea.ac.kr

^e Department of Chemical Engineering and Materials Science, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Ettimadai, Coimbatore 641112, India

Abstract

A bioreductant-resistant ‘turn-on’ chemodosimetric fluorescent probe Mito-1 has been developed for the detection of mitochondrial HNO in live cells. Mito-1 enables the detection of HNO as low as ∼18 nM. It has the capability to detect both exogenous and endogenous mitochondrial HNO formations in cellular milieus by providing fluorescence images. Its two-photon imaging ability fosters its use as a noninvasive imaging tool for the detection of mitochondrial nitroxyl.

This article is part of the themed collections: Biological optical imaging and Most downloaded articles of 2017: Analytical Chemistry

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Article information

DOI: https://doi.org/10.1039/C6CC09082F
Article type: Communication
Submitted: 14 Nov 2016
Accepted: 10 Jän 2017
First published: 10 Jän 2017

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Chem. Commun., 2017,53, 1723-1726

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A bioorthogonal ‘turn-on’ fluorescent probe for tracking mitochondrial nitroxyl formation

K. Sunwoo, K. N. Bobba, J. Lim, T. Park, A. Podder, J. S. Heo, S. G. Lee, S. Bhuniya and J. S. Kim, Chem. Commun., 2017, 53, 1723 DOI: 10.1039/C6CC09082F

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A bioorthogonal ‘turn-on’ fluorescent probe for tracking mitochondrial nitroxyl formation†

Abstract

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A bioorthogonal ‘turn-on’ fluorescent probe for tracking mitochondrial nitroxyl formation

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