Issue 6, 2021

Clickable gold-nanoparticles as generic probe precursors for facile photoaffinity labeling application

Abstract

Rapid access to appropriately functionalized probes is crucial in chemical labeling approaches to target identification studies. We designed and synthesized clickable gold-nanoparticles as generic probe precursors that enable (1) one-step ligand derivatization by click chemistry, and (2) facile photoaffinity labeling application. Using cholesterol as a model ligand, we successfully demonstrated the utility of the ligand-clicked probe in photoaffinity labeling of endogenously expressed oxysterol-binding protein (OSBP) in cell lysate.

Graphical abstract: Clickable gold-nanoparticles as generic probe precursors for facile photoaffinity labeling application

Supplementary files

Article information

Article type
Communication
Submitted
15 Eost 2020
Accepted
08 Ker. 2020
First published
17 Ker. 2020

Org. Biomol. Chem., 2021,19, 1268-1273

Clickable gold-nanoparticles as generic probe precursors for facile photoaffinity labeling application

K. Mori and K. Sakurai, Org. Biomol. Chem., 2021, 19, 1268 DOI: 10.1039/D0OB01688H

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