Issue 26, 2022

Cadherin-dependent adhesion modulated 3D cell-assembly

Abstract

The emergence of synthetic biology has opened new avenues in constructing cell-assembly biosystems with specific gene expression and function. The phenomena of cell spreading and detachment during tissue development and cancer metastasis are caused by surface tension, which in turn results from differences in cell–cell adhesion mediated by the dimerization of cadherin expressed on the cell surface. In this study, E- and P-cadherin plasmids were first constructed based on the differential adhesion hypothesis, then they were electroporated into K562 cells and HEK293T cells, respectively, to explore the process of cell migration and assembly regulated by cadherins. Using this approach, some special 3D cell functional components with a phase separation structure were fabricated successfully. Our work will be of potential application in the construction of self-assembling synthetic tissues and organoids.

Graphical abstract: Cadherin-dependent adhesion modulated 3D cell-assembly

Supplementary files

Article information

Article type
Paper
Submitted
09 মে 2022
Accepted
08 জুন 2022
First published
08 জুন 2022

J. Mater. Chem. B, 2022,10, 4959-4966

Cadherin-dependent adhesion modulated 3D cell-assembly

W. Han, M. He, Y. Zhang, J. Zhou, Z. Li, X. Liu, X. Sun, X. Yin, D. Yao and H. Liang, J. Mater. Chem. B, 2022, 10, 4959 DOI: 10.1039/D2TB01006B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements