An efficient dye-sensitized NIR emissive lanthanide nanomaterial and its application in fluorescence-guided peritumoral lymph node dissection

Abstract

The luminescence intensity of near-infrared (NIR) emitting lanthanide nanoparticles (LnNPs) is usually limited, owing to their small absorption cross section. Although dye sensitization has been proven to be an effective way to improve the luminescence intensity of LnNPs, the sensitization effect is fairly limited, owing to the simplicity of the sensitizers used and the complexity of the energy transfer process, typically involving three steps. In this study, a more efficient sensitizer (Cy7) was chosen to replace a commonly used one (ICG) and the energy transfer process was also optimized through using Yb³⁺ ions as emitter ions and Nd³⁺ ions as intermediate ions. With Cy7 as a sensitizer, the sensitization effect was assessed to be better than with ICG, owing to the higher quantum yield of Cy7. Meanwhile, the Cy7-sensitized NIR lanthanide nanomaterial was proven to be good for deep tissue penetration and low-power excitation bioimaging. Furthermore, the highly-enhanced NIR signal was successfully used in blood vessel imaging and fluorescence-guided peritumoral lymph node dissection in a mouse model.