Issue 36, 2019

Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis

Abstract

Multi-dimensional gene analysis provides in-depth insights into gene sequence, locus variations and molecular abundance, whereas it is vulnerable to the perturbation of complex reaction networks and always compromises on the discrimination of analogous sequences. Here, we present a sequence oriented enrichment method patterned by the prescribed locus without crosstalk between concurrent reactions. Energetically favourable structures of nucleic acid probes are theoretically derived and oriented to a specific gene locus. We designed a pair of universal probes for multiple conserved loci to avoid side reactions from undesired interactions among increased probe sets. Furthermore, competitive probes were customized to sink analogues for differentiating the reaction equilibrium and kinetics of sequence enrichment from the target, so variant loci can be synchronously identified with nucleotide-level resolution. Thus, the gene locus guides sequence enrichment and combinatorial signals to create unique codes, which provides access to multidimensional and precise information for gene decoding.

Graphical abstract: Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis

Supplementary files

Article information

Article type
Edge Article
Submitted
22 may 2019
Accepted
22 iyl 2019
First published
22 iyl 2019
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2019,10, 8421-8427

Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis

Y. Zhao, X. Fang, F. Chen, M. Bai, C. Fan and Y. Zhao, Chem. Sci., 2019, 10, 8421 DOI: 10.1039/C9SC02496D

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