Issue 39, 2021

A highly selective ratiometric molecular probe for imaging peroxynitrite during drug-induced acute liver injury

Abstract

Drug-induced acute liver injury (DIALI) is a common liver disease, affecting a number of people worldwide with increasing morbidity each year. Thus, it is vital to develop new tools for intervention and diagnosis. Peroxynitrite (ONOO), a highly reactive species, plays an important role in the DIALI process. Thus, in situ molecular imaging of endogenous ONOO levels is considerably significant for detecting ONOO. In this work, we present two destroyed-type ratiometric fluorescent probes, AHC and AHMC, for ONOO detection by using a molecular hybridization strategy. The probe AHMC was developed by introducing the ester structure to AHC directly to enhance its membrane penetrability for living cell imaging. Probe AHC exhibited good analytical performance toward ONOO compared to other reactive species, with a low detection limit (≈1.8 nM) and a strong ratiometric fluorescence response (134-fold). In cell imaging experiments, AHMC showed outstanding selectivity, favourable biocompatibility and mitochondria-targeting ability, which not only was used to detect endogenous and exogenous ONOO changes, but also enabled noninvasive visualization of ONOO generation in a different drug-induced DIALI model. We hope that these ratiometric probes can be useful chemical tools for the in-depth research of drug-induced acute hepatotoxicity.

Graphical abstract: A highly selective ratiometric molecular probe for imaging peroxynitrite during drug-induced acute liver injury

Supplementary files

Article information

Article type
Paper
Submitted
13 iyl 2021
Accepted
18 avq 2021
First published
19 avq 2021

J. Mater. Chem. B, 2021,9, 8246-8252

A highly selective ratiometric molecular probe for imaging peroxynitrite during drug-induced acute liver injury

X. Gong, D. Cheng, W. Li, Y. Shen, R. Peng, L. Shi, L. He and L. Yuan, J. Mater. Chem. B, 2021, 9, 8246 DOI: 10.1039/D1TB01534F

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