Issue 91, 2021

Visualization of lipophagy using a supramolecular FRET pair

Abstract

A rationally designed supramolecular FRET pair consisting of cyanine3-cucurbit[7]uril (Cy3-CB[7]) and boron-dipyrromethene 630/650-adamantylammonium (BDP-AdA) can be used to visualize organelle-specific autophagy events. The intracellular accumulations of Cy3-CB[7] in lysosomes and BDP-AdA in lipid droplets (LDs) and the formation of an intracellular host–guest complex between Cy3-CB[7] and BDP-AdA resulting in FRET signals allow us to visualize the fusion of LDs with lysosomes, namely, lipophagy. This study demonstrates the potential of supramolecular imaging based on bio-orthogonal host–guest interactions in the investigation of selective autophagy events.

Graphical abstract: Visualization of lipophagy using a supramolecular FRET pair

Supplementary files

Article information

Article type
Communication
Submitted
27 Leq 2021
Accepted
26 Dit 2021
First published
27 Dit 2021

Chem. Commun., 2021,57, 12179-12182

Visualization of lipophagy using a supramolecular FRET pair

A. Lee, M. Li, Y. H. Ko, S. Park, J. Seo, K. M. Park and K. Kim, Chem. Commun., 2021, 57, 12179 DOI: 10.1039/D1CC04779E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements