Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR†
Abstract
The progress of a polymerase chain reaction (PCR) was sensitively monitored based on the increase in fluorescence of N,N′-bis(3-aminopropyl)-2,7-diamino-1,8-naphthyridine, which was covalently anchored on the cytosine bulge directly neighbouring the 5′-T_G-3′/5′-CCA-3′ sequence in the hairpin tag at the 5′ end of the PCR primer.