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Issue 16, 2014
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On the importance of image formation optics in the design of infrared spectroscopic imaging systems

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Abstract

Infrared spectroscopic imaging provides micron-scale spatial resolution with molecular contrast. While recent work demonstrates that sample morphology affects the recorded spectrum, considerably less attention has been focused on the effects of the optics, including the condenser and objective. This analysis is extremely important, since it will be possible to understand effects on recorded data and provides insight for reducing optical effects through rigorous microscope design. Here, we present a theoretical description and experimental results that demonstrate the effects of commonly-employed cassegranian optics on recorded spectra. We first combine an explicit model of image formation and a method for quantifying and visualizing the deviations in recorded spectra as a function of microscope optics. We then verify these simulations with measurements obtained from spatially heterogeneous samples. The deviation of the computed spectrum from the ideal case is quantified via a map which we call a deviation map. The deviation map is obtained as a function of optical elements by systematic simulations. Examination of deviation maps demonstrates that the optimal optical configuration for minimal deviation is contrary to prevailing practice in which throughput is maximized for an instrument without a sample. This report should be helpful for understanding recorded spectra as a function of the optics, the analytical limits of recorded data determined by the optical design, and potential routes for optimization of imaging systems.

Graphical abstract: On the importance of image formation optics in the design of infrared spectroscopic imaging systems

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Publication details

The article was received on 06 Sep 2013, accepted on 21 Apr 2014 and first published on 22 Apr 2014


Article type: Paper
DOI: 10.1039/C3AN01687K
Author version available: Download Author version (PDF)
Citation: Analyst, 2014,139, 4031-4036
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    On the importance of image formation optics in the design of infrared spectroscopic imaging systems

    D. Mayerich, T. van Dijk, M. J. Walsh, M. V. Schulmerich, P. S. Carney and R. Bhargava, Analyst, 2014, 139, 4031
    DOI: 10.1039/C3AN01687K

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