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Issue 24, 2018
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An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection

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Abstract

Gene point mutations are commonly associated with the development of cancer and other diseases. The rapid and accurate detection of mutation is increasingly needed in personalized therapeutic regimes. In this proof-of-concept study, an isothermal single base extension (SBE) reaction was developed for the simple and fast amplification of mutations. Combined with a lateral flow biosensor and electrochemical assay, a limit of detection of 1 nM and 1 pM were achieved, respectively. Compared with traditional PCR-based assays, this approach is faster, simpler and more cost-effective. Furthermore, the reduced experimental requirements enable its integration with portable micro-fluidic devices, forming a simple and automatic point of care device for point mutation detections.

Graphical abstract: An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection

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Publication details

The article was received on 04 Apr 2018, accepted on 21 Apr 2018 and first published on 24 Apr 2018


Article type: Paper
DOI: 10.1039/C8AY00746B
Citation: Anal. Methods, 2018,10, 2863-2868
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    An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection

    N. Sun, Q. Guo, X. Li, J. Chen, X. Liu, K. Wong and Z. Fang, Anal. Methods, 2018, 10, 2863
    DOI: 10.1039/C8AY00746B

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