Jump to main content
Jump to site search


An aptamer-patterned hydrogel for the controlled capture and release of proteins via biorthogonal click chemistry and DNA hybridization

Author affiliations

Abstract

Three-dimensional (3D) bioactive protein-patterned hydrogels provide a more biomimetic environment for cell culture than traditional bioinert scaffolds. However, it has proved difficult to obtain a hydrogel with simultaneously patterned proteins and controlled release at a later stage without inactivating the bioactivity of the fragile proteins. In this study, an aptamer-patterned PEG hydrogel was formed using biorthogonal “click chemistry”. The patterned aptamer could selectively capture the bioactive protein based on the specific affinity between the aptamer and protein, which results in the formation of a patterned protein within the hydrogel. More importantly, by adding the complementary DNA strand (cDNA) of the aptamer, the protein can dissociate from the aptamer at any desired time and concentration due to the DNA hybridization. This aptamer-patterned hydrogel could become a smart and biomimetic 3D micro-environment for controlled delivery of proteins simultaneously in a spatiotemporal manner.

Graphical abstract: An aptamer-patterned hydrogel for the controlled capture and release of proteins via biorthogonal click chemistry and DNA hybridization

Back to tab navigation

Supplementary files

Publication details

The article was received on 31 Mar 2017, accepted on 27 Jun 2017 and first published on 28 Jun 2017


Article type: Paper
DOI: 10.1039/C7TB00883J
Citation: J. Mater. Chem. B, 2017, Advance Article
  •   Request permissions

    An aptamer-patterned hydrogel for the controlled capture and release of proteins via biorthogonal click chemistry and DNA hybridization

    Z. Zhang, J. Du, Y. Li, J. Wu, F. Yu and Y. Chen, J. Mater. Chem. B, 2017, Advance Article , DOI: 10.1039/C7TB00883J

Search articles by author

Spotlight

Advertisements