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RNA-directed off/on switch of RNase H activity using boronic ester formation

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Abstract

RNase H is a non-specific endonuclease which degrades selectively the RNA strand in DNA/RNA duplexes. We demonstrate in the present study that 5′-boronic acid modified oligonucleotides hybridized to a RNA target sequence converts RNase H to an inactivated enzyme complex. The dynamic formation of a boronate ester upon addition of a diol moiety disrupts the enzyme-inhibitor complex and reactivates RNase H. Moreover, we show that reactivation of RNase H function can also be engineered through short RNA trimers inputs that fashion RNase H from a non-specific DNA-guided enzyme into an informational and programmable RNA-guided one. Examples of programmable RNA recognition and cleavage illustrate the potential of this new stimuli-responsive system.

Graphical abstract: RNA-directed off/on switch of RNase H activity using boronic ester formation

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Publication details

The article was received on 28 Aug 2017, accepted on 11 Sep 2017 and first published on 12 Sep 2017


Article type: Paper
DOI: 10.1039/C7OB02145C
Citation: Org. Biomol. Chem., 2017, Advance Article
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    RNA-directed off/on switch of RNase H activity using boronic ester formation

    M. Reverte, I. Barvik, J. Vasseur and M. Smietana, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB02145C

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