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Issue 16, 2017
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Synthesis of paramagnetic dendritic silica nanomaterials with fibrous pore structure (Fe3O4@KCC-1) and their application in immobilization of lipase from Candida rugosa with enhanced catalytic activity and stability

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Abstract

Paramagnetic mesoporous fibrous silica (Fe3O4@KCC-1) was prepared and its surface was functionalized with 3-aminopropyltriethoxysilane (APTES) to obtain amino functionalized magnetic nanoparticles. Lipase from Candida rugosa was immobilized on functionalized magnetite Fe3O4@KCC-1-NH2 using glutaraldehyde (GLU) as the linker. The nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), powder X-ray diffraction (XRD), vibrating sample magnetometry (VSM) and Fourier transformed infrared spectroscopy (FTIR). The characterization results revealed successful immobilization of lipase on functionalized magnetite with a saturation magnetization of 62.73 and 42.65 emu g−1 for magnetic nanoparticles and carbonyl functionalized carriers, respectively. The applied approach for support preparation, activation, and optimization of immobilization conditions, with a good lipase loading of 283 mg g−1 of carrier, provided better resistance to temperature and pH inactivation than free lipase and hence expanded the reaction pH and temperature regions, with an optimum pH of 6 and temperature of 35 °C. Immobilized Lipase Candida rugosa (ICRL) showed enzyme activity of 630 U g−1, maintained above 560.7 U g−1 (89%) of the initial activity after 28 days and 434.7 U g−1 (69%) after 10 cycles. Thus, ICRL showed improved storage stability and reusability.

Graphical abstract: Synthesis of paramagnetic dendritic silica nanomaterials with fibrous pore structure (Fe3O4@KCC-1) and their application in immobilization of lipase from Candida rugosa with enhanced catalytic activity and stability

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Publication details

The article was received on 31 May 2017, accepted on 28 Jun 2017 and first published on 28 Jun 2017


Article type: Paper
DOI: 10.1039/C7NJ01912B
Citation: New J. Chem., 2017,41, 8222-8231
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    Synthesis of paramagnetic dendritic silica nanomaterials with fibrous pore structure (Fe3O4@KCC-1) and their application in immobilization of lipase from Candida rugosa with enhanced catalytic activity and stability

    Z. Ali, L. Tian, B. Zhang, N. Ali, M. khan and Q. Zhang, New J. Chem., 2017, 41, 8222
    DOI: 10.1039/C7NJ01912B

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