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Issue 51, 2017
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The efficient synthesis and purification of amyloid-β(1–42) using an oligoethylene glycol-containing photocleavable lysine tag

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Abstract

A short, monodisperse oligoethylene glycol-containing photocleavable lysine tag was developed to facilitate the efficient purification of hydrophobic and fibril-forming peptides. This new tag was used to prepare a modified Aβ42 peptide with increased solubility and decreased propensity to aggregate in aqueous media. The solubilising tag was readily removed by irradiation with UV light and permitted the preparation and isolation of Aβ42 in high purity and yield.

Graphical abstract: The efficient synthesis and purification of amyloid-β(1–42) using an oligoethylene glycol-containing photocleavable lysine tag

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Publication details

The article was received on 24 Apr 2017, accepted on 06 Jun 2017 and first published on 06 Jun 2017


Article type: Communication
DOI: 10.1039/C7CC03147E
Citation: Chem. Commun., 2017,53, 6903-6905
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    The efficient synthesis and purification of amyloid-β(1–42) using an oligoethylene glycol-containing photocleavable lysine tag

    J. A. Karas, A. Noor, C. Schieber, T. U. Connell, F. Separovic and P. S. Donnelly, Chem. Commun., 2017, 53, 6903
    DOI: 10.1039/C7CC03147E

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