Issue 30, 2017
From the journal:

Chemical Communications

An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins†

Kai Cai,‡ab   Ying Tan,‡ab   Chunyan Tan, ORCID logo *ab   Jiatao Wu,ab   Pan Wu,ab   Jiamei Liang,ab   Shuwen Liu,ab   Bibo Zhangab  and  Yuyang Jiangbc  

* Corresponding authors

a Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

b The State Key Laboratory Breeding Base-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P. R. China
E-mail: tancy@sz.tsinghua.edu.cn
Tel: +86-755-26036533

c School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, P. R. China

Abstract

The iminodiacetate–Ni2+–hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization of hexahistidine-tagged (His-tagged) proteins using PPEIDA was demonstrated.

Graphical abstract: An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins

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Article information

DOI
https://doi.org/10.1039/C7CC00850C
Article type
Communication
Submitted
01 Feb 2017
Accepted
27 Feb 2017
First published
27 Feb 2017

Chem. Commun., 2017,53, 4191-4194

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An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins

K. Cai, Y. Tan, C. Tan, J. Wu, P. Wu, J. Liang, S. Liu, B. Zhang and Y. Jiang, Chem. Commun., 2017, 53, 4191 DOI: 10.1039/C7CC00850C

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