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An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins

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Abstract

The iminodiacetate–Ni2+–hexahistidine system is extensively used in protein purification. In this study, an anionic conjugated polyelectrolyte (CPE) with a poly(p-phenylene ethynylene) backbone and iminodiacetate side chains, named PPEIDA, was designed and synthesized. Recognition and visualization of hexahistidine-tagged (His-tagged) proteins using PPEIDA was demonstrated.

Graphical abstract: An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins

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Publication details

The article was received on 01 Feb 2017, accepted on 27 Feb 2017, published on 27 Feb 2017 and first published online on 27 Feb 2017


Article type: Communication
DOI: 10.1039/C7CC00850C
Citation: Chem. Commun., 2017, Advance Article
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    An iminodiacetate-modified conjugated polyelectrolyte for fluorescent labeling of histidine-tagged proteins

    K. Cai, Y. Tan, C. Tan, J. Wu, P. Wu, J. Liang, S. Liu, B. Zhang and Y. Jiang, Chem. Commun., 2017, Advance Article , DOI: 10.1039/C7CC00850C

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