Jump to main content
Jump to site search


Anchoring β-cyclodextrin modified lysine to polymer monolith with biotin: specific capture of plasminogen

Abstract

Plasminogen (Plg) is a kind of glycoprotein which plays an important role in cell migration. The determination of Plg content can directly reflect the abnormality manifestation of fibrinolytic system dysfunctions. In the present work, lysine (Lys)-based adsorbents were prepared for specific capture of Plg through the covalent binding of Lys with polymer monolithic substrate. Lys was modified with β-cyclodextrin (β-CD) via a click reaction and anchored to the substrate with biotin by host-guest interaction. The biotin-Lys-CD based monolithic material was employed for the specific capture of Plg. Combing with mass spectrometry (MS) determinations, the method exhibited a low detection limit of 1.0 fmol with relative standard deviations below 10.0% for Plg. Considering the advantages of simplicity, sensitivity, and high specificity, the developed approach was successfully applied to the determination of Plg in human plasma samples and opened a gallery for testing Plg as a biomarker for the diagnosis of fibrinolytic system dysfunctions.

Back to tab navigation

Publication details

The article was received on 30 Aug 2017, accepted on 07 Nov 2017 and first published on 07 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN01436H
Citation: Analyst, 2017, Accepted Manuscript
  •   Request permissions

    Anchoring β-cyclodextrin modified lysine to polymer monolith with biotin: specific capture of plasminogen

    H. Zheng, N. Song, X. Li and Q. Jia, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01436H

Search articles by author

Spotlight

Advertisements