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Issue 44, 2016
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Flexibility is important for inhibition of the MDM2/p53 protein–protein interaction by cyclic β-hairpins

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Abstract

Protein–protein interactions that have large, flat and featureless binding sites are difficult drug targets. In the development of their modulators conventional drug discovery strategies are often unsuccessful. Gaining a detailed understanding of the binding mode of protein–protein interaction inhibitors is therefore of vast importance for their future pharmaceutical use. The MDM2/p53 protein pair is a highly promising target for cancer treatment. Disruption of the protein complex using p53 α-helix mimetics has been shown to be a successful strategy to control p53 activity. To gain further insight into the binding of inhibitors to MDM2, the flexibility of four cyclic β-hairpins that act as α-helical mimetics and potential MDM2/p53 interaction inhibitors was investigated in relation to their inhibitory activity. MDM2-binding of the mimetics was determined using fluorescence polarization and surface plasmon resonance assays, whereas their conformation and dynamics in solution was described by the combined experimental and computational NAMFIS analysis. Molecular flexibility was shown to be important for the activity of the cyclic β-hairpin based MDM2 inhibitors.

Graphical abstract: Flexibility is important for inhibition of the MDM2/p53 protein–protein interaction by cyclic β-hairpins

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Publication details

The article was received on 15 Jul 2016, accepted on 05 Oct 2016, published on 05 Oct 2016 and first published online on 05 Oct 2016


Article type: Paper
DOI: 10.1039/C6OB01510G
Citation: Org. Biomol. Chem., 2016,14, 10386-10393
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    Flexibility is important for inhibition of the MDM2/p53 protein–protein interaction by cyclic β-hairpins

    E. Danelius, M. Pettersson, M. Bred, J. Min, M. B. Waddell, R. K. Guy, M. Grøtli and M. Erdelyi, Org. Biomol. Chem., 2016, 14, 10386
    DOI: 10.1039/C6OB01510G

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