Jump to main content
Jump to site search

Issue 8, 2016
Previous Article Next Article

Modern mass spectrometry for synthetic biology and structure-based discovery of natural products

Author affiliations

Abstract

Covering: up to 2016

In this highlight, we describe the current landscape for dereplication and discovery of natural products based on the measurement of the intact mass by LC-MS. Often it is assumed that because better mass accuracy (provided by higher resolution mass spectrometers) is necessary for absolute chemical formula determination (≤1 part-per-million), that it is also necessary for dereplication of natural products. However, the average ability to dereplicate tapers off at ∼10 ppm, with modest improvement gained from better mass accuracy when querying focused databases of natural products. We also highlight some recent examples of how these platforms are applied to synthetic biology, and recent methods for dereplication and correlation of substructures using tandem MS data. We also offer this highlight to serve as a brief primer for those entering the field of mass spectrometry-based natural products discovery.

Graphical abstract: Modern mass spectrometry for synthetic biology and structure-based discovery of natural products

Back to tab navigation

Publication details

The article was received on 27 Feb 2016 and first published on 04 Jul 2016


Article type: Highlight
DOI: 10.1039/C6NP00024J
Citation: Nat. Prod. Rep., 2016,33, 942-950
  •   Request permissions

    Modern mass spectrometry for synthetic biology and structure-based discovery of natural products

    M. T. Henke and N. L. Kelleher, Nat. Prod. Rep., 2016, 33, 942
    DOI: 10.1039/C6NP00024J

Search articles by author

Spotlight

Advertisements