Jump to main content
Jump to site search

Issue 24, 2016
Previous Article Next Article

Spatiotemporal control of kinesin motor protein by photoswitches enabling selective single microtubule regulations

Author affiliations

Abstract

Artificial control of bio-nanomachines should have a major impact on the development of controllable transport systems for specific cargo transport on chips. Precise spatiotemporal control and local regulation of the bio-motor activity will, however, be necessary if we are to accomplish such a goal. In this study, we exploited the photoswitching properties of azobenzene-based high-energy molecules and inhibitors to control a single kinesin-driven microtubule that has potential to work as a nanocarrier for molecular cargos. In particular, we could influence the local concentration and dispersion of the microtubules at any desired position and time by irradiating a local area of the motility system at one wavelength, while irradiating the entire area at another wavelength, to enrich either cis or trans isomers of photoswitches in the selected region. Furthermore, various regulations (e.g., transporting, bending, breaking) of single microtubules were possible while almost arresting ambient microtubules—all without the need for any surface patterning.

Graphical abstract: Spatiotemporal control of kinesin motor protein by photoswitches enabling selective single microtubule regulations

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 31 Aug 2016, accepted on 19 Oct 2016 and first published on 21 Oct 2016


Article type: Paper
DOI: 10.1039/C6LC01098A
Citation: Lab Chip, 2016,16, 4702-4709
  • Open access: Creative Commons BY license
  •   Request permissions

    Spatiotemporal control of kinesin motor protein by photoswitches enabling selective single microtubule regulations

    K. R. S. Kumar, A. S. Amrutha and N. Tamaoki, Lab Chip, 2016, 16, 4702
    DOI: 10.1039/C6LC01098A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author