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Issue 21, 2015
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Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding

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Abstract

Phage display antibodies are widely used to follow heparan sulfate (HS) expression in tissues and cells. We demonstrate by ELISA, that cations alter phage display antibody binding profiles to HS and this is mediated by changes in polysaccharide conformation, demonstrated by circular dichroism spectroscopy. Native HS structures, expressed on the cell surfaces of neuroblastoma and fibroblast cells, also exhibited altered antibody binding profiles following exposure to low mM concentrations of these cations. Phage display antibodies recognise conformationally-defined HS epitopes, rather than sequence alone, as has been assumed, and resemble proteins in being sensitive to changes in both charge distribution and conformation following binding of cations to HS polysaccharides.

Graphical abstract: Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding

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Publication details

The article was received on 20 Mar 2015, accepted on 28 Apr 2015 and first published on 28 Apr 2015


Article type: Paper
DOI: 10.1039/C5OB00564G
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2015,13, 6066-6072
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    Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding

    V. Solari, T. R. Rudd, S. E. Guimond, A. K. Powell, J. E. Turnbull and E. A. Yates, Org. Biomol. Chem., 2015, 13, 6066
    DOI: 10.1039/C5OB00564G

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