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Issue 10, 2014
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A functional microengineered model of the human splenon-on-a-chip

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Abstract

The spleen is a secondary lymphoid organ specialized in the filtration of senescent, damaged, or infected red blood cells. This unique filtering capacity is largely due to blood microcirculation through filtration beds of the splenic red pulp in an open-slow microcirculation compartment where the hematocrit increases, facilitating the recognition and destruction of unhealthy red blood cells by specialized macrophages. Moreover, in sinusal spleens such as those of humans, blood in the open-slow microcirculation compartment has a unidirectional passage through interendothelial slits before reaching the venous system. This further physical constraint represents a second stringent test for erythrocytes ensuring elimination of those cells lacking deformability. With the aim of replicating the filtering function of the spleen on a chip, we have designed a novel microengineered device mimicking the hydrodynamic forces and the physical properties of the splenon, the minimal functional unit of the red pulp able to maintain filtering functions. In this biomimetic platform, we have evaluated the mechanical and physiological responses of the splenon using human red blood cells and malaria-infected cells. This novel device should facilitate future functional studies of the spleen in relation to malaria and other hematological disorders.

Graphical abstract: A functional microengineered model of the human splenon-on-a-chip

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Publication details

The article was received on 28 Dec 2013, accepted on 11 Feb 2014 and first published on 12 Feb 2014


Article type: Paper
DOI: 10.1039/C3LC51449H
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2014,14, 1715-1724
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    A functional microengineered model of the human splenon-on-a-chip

    L. G. Rigat-Brugarolas, A. Elizalde-Torrent, M. Bernabeu, M. De Niz, L. Martin-Jaular, C. Fernandez-Becerra, A. Homs-Corbera, J. Samitier and H. A. del Portillo, Lab Chip, 2014, 14, 1715
    DOI: 10.1039/C3LC51449H

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